This process is called formation of clamp connections. First, a bulging pocket
forms in the hyphal cell wall at a point between the two nuclei,
a and
b. Both nuclei then divide mitotically. These divisions are oriented such
that the
a' nucleus is positioned in the clamp pocket and both the
a" and
b' nuclei are toward the tip of the hypha, while the
b" nucleus takes a posterior position. Next, a cell wall forms between
the clamp, the posterior cell and the tip of the hypha, leaving the tip a
complete cell with two nuclei. Finally, the clamp curves back toward the hypha
and merges with the posterior cell to create another binucleate cell.