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2' deoxyribonucleoside triphosphate
The building blocks of DNA replication. A five-membered, oxygen-containing
ribose sugar ring that has three phosphate groups attached to its 5' carbon and
either an adenine, cytosine, guanine, or thymine base group attached to its 1'
carbon.
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Base-pair excision
One class of DNA repair system. Recognizes and removes single nucleotide
mutations that result from unnatural bases.
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Daughter strand
Refers to the newly synthesized strand of DNA that is copied via the addition of
complementary
nucleotides
from one strand of
pre-existing DNA during DNA replication.
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DNA Helicase
The enzyme responsible for separating the two strands of DNA in a helix so that
they can be copied during DNA replication.
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DNA Ligase
The enzyme responsible for sealing together breaks or nicks in a DNA strand.
Responsible for patching together Okazaki fragments on the lagging strand
during DNA replication.
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DNA Polymerase
The enzyme responsible for catalyzing the addition of
nucleotide
substrates to DNA both during and
after DNA replication.
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Primase
The enzyme responsible for initiating synthesis of RNA primers on the lagging
strand during DNA replication.
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Holoenzyme
A term used to describe a collection of different enzymes that work together in
a given process such as DNA replication.
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Hydrolysis
The process in which water is chemically added to a molecule.
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Lagging strand
In DNA replication, the strand of pre-existing DNA that is oriented in the 5' to
3' direction with respect to the direction of replication on which synthesis is
discontinuous.
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Leading strand
In DNA replication, the strand of pre-existing DNA that is oriented in the 3' to
5' direction with respect to the direction of replication on which replication
is continuous.
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Mismatch repair
One class of DNA repair system. Recognizes and removes mutations that result
from base-pairing that is not
complementary.
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Okazaki fragment
Short stretches of newly synthesized DNA found on the lagging strand during
DNA replication.
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Origin of replication
Site of initiation of DNA replication. Short, usually internal stretch in a DNA
helix that opens so that each strand is separate for DNA replication.
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Parent strand
In DNA replication, refers to the pre-existing single strand of DNA that is
copied into a new strand of DNA via
complementary
base pairing.
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Pyrophosphate
A two phosphate-containing molecule. In DNA replication, it is released from a
2' deoxyribonucleoside triphosphate during its addition to a growing, newly
synthesized DNA strand. Its subsequent hydrolysis provides the energy for
the addition reaction.
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Replication fork
Term used to describe the junction at which nucleotide substrates are being
added to a growing DNA chain during DNA replication. Its shape resembles a "Y"
where the two branches represent single stranded daughter strands of DNA and
the base represents helical DNA.
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RNA Primer
Short stretches of ribonucleotides (RNA substrates) found on the lagging
strand during DNA replication. Helps initiate lagging strand replication and
are later removed.
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Semi-conservative
Refers to the fact that after the replication of one DNA helix each of the two
daughter helices that result contain one newly-synthesized and one pre-
existing strand of DNA.
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Short-patch excision
One class of DNA repair system. Recognizes and removes short stretches of DNA
that surround mutations resulting from large adducts on a DNA strand that impede
DNA replication.
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Single-stranded binding protein
A protein involved in helping to keep strands of DNA that have been separated by
DNA helicase from recoiling in a helix. It works by coating the single
strands in such a way as not to cover the bases, allowing them to remain free
for base pairing.
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Thymine dimer
A form of DNA damage that results from radiation. Adjacent thymines on the same
strand of DNA form a bond that results in a bulky adduct that can impede DNA
replication.
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Tautomerization
A process in which a molecule undergoes an electron rearrangement that results
in a slightly different organization of the same molecule. The two forms of the
same molecule are called "tautomers" of each other.
