- The Mannon's daughter. Lavinia is wooden, stiff-shouldered daughter, flat- chested, thin, angular and dressed in simple black. She shares her mother Christine's lustrous copper hair and mask-like face. The severe Lavinia considers herself robbed of love at her mother's hands. Thus she schemes to take Christine's place and become the wife of her father and mother of her brother. She ultimately does so upon her mother's death, reincarnating her in her own flesh.
in-depth analysis of Lavinia Mannon.
- A striking woman of forty with a fine, voluptuous figure, flowing animal grace, and a mass of beautiful copper hair. She wears green, which symbolizes her envy. Her pale face is also a life-like mask, a mask that represents both her duplicity and her almost super-human efforts at repression. Having long abhorred her husband Ezra, Christine plots his murder with her lover Brant upon his return from the Civil War.
in-depth analysis of Christine Mannon.
- The Mannon son returned from war. Orin bears a striking resemblance to his father and Captain Brant, though he appears as a weakened, refined, and oversensitive version of each. He possesses a boyish charm that invites the maternal favors of women. He loves his mother incestuously, flying into a jealous rage upon the discovery of her love affair that leads to Christine and Brant's deaths. Orin will then force he and his sister to judgment for their crimes in an attempt to rejoin his mother in death.
in-depth analysis of Orin Mannon.
Brigadier-General Ezra Mannon
- The great Union general. Ezra is a spare, big-boned man of exact and wooden movements. His mannerisms suggest the statue-like poses of military heroes. His brusque and authoritative voice has a hollow and repressed quality. As his near- homophonic name suggests, he is Agamemnon's counterpart, the general returned from war to be murdered by his wife and her lover. He continues to exert his influence in symbolic form. His various images, and his portrait in particular, call his family to judgment from beyond the grave.
Captain Adam Brant
- A powerful, romantic sea captain. Brant has a swarthy complexion, sensual mouth, and long, coal-black hair. He also of course bares a striking resemblance to the other Mannon men, sharing their same, mask-like faces. The child of the illegitimate Mannon line, he returns to wreak vengeance on Ezra's household. He steals Ezra's wife, a woman he imagines in the image of his mother, and seduces Lavinia to conceal their affair.
- A longtime friend of the Mannon children. Hazel is a pretty, healthy, dark- haired girl of nineteen. O'Neill describes her character as frank, innocent, amiable, and good. She functions as Orin's would-be sweetheart, and both Christine and Lavinia attempting to pass Orin off onto her so they can flee with their suitors. Hazel also haplessly attempts to rescue Orin from his fate.
Captain Peter Niles
- An artillery captain for the Union. Peter resembles his sister in character. He is straightforward, guileless, and good-natured, failing to apprehend the machinations afoot in the Mannon house until the very end of the trilogy. He functions as the suitor Lavinia first rejects and later takes up as a substitute for Captain Brant.
- The Mannons' aged gardener. Seth is stoop-shoulded and raw-boned but still strong. Like his employers, his gaunt face gives the impression of a life-like mask. In his time with the Mannons, he has learned most of the family's secrets and colluded in keeping them. A watchman figure of sorts, he is repeatedly seen wandering the grounds and singing the sea chanty "Shenandoah."
- A fat carpenter in his fifties. Ames is a typical and relatively benign town gossip-monger.
- Amos's wife. Louisa is similarly a gossip though much more maliciously.
- Louisa's meek middle-aged cousin and most eager listener.
- A small, wizened man of sixty. Borden is the shrewd manager of the Mannon shipping company.
- Josiah's wife. Emma is a typical New England woman of pure English ancestry, with a horse face, buckteeth, and big teeth. Her manner is defensively sharp and assertive.
Everett Hills, D.D.
- The well-fed minister of a prosperous small town: snobbish, unctuous, and ingratiating in his demeanor.
- A sallow, flabby, and self-effacing minister's wife.
Dr. Joseph Blake
- The Mannon's kindly family physician, stout, self-important, and stubbornly opinionated.
- A drunk, weather-beaten man of sixty-five. Though dissipated, he possesses a romantic, troubadour-of-the-sea air. Critic Travis Bogard considers his cameo appearance in "The Hunted" as O'Neill's farewell to the seaman heroes of his earlier plays.
- The shrill, goat-bearded clerk of the town hardware store who breaks into the Mannon house on a wager.
- A sly, cackling farmer who helps goad Small into the house.
- A fat, boisterous Portuguese fishing captain who also helps goad Small into the house.