Pozzo enters, driving Lucky ahead of him by a rope around his neck. Vladimir and Estragon wonder if Pozzo is Godot, but he tells them that he is Pozzo and asks if they have heard of him. They tell him that they have not. Pozzo commands Lucky to put down his stool, and sits down and begins to eat some chicken. While he eats, Vladimir and Estragon circle around Lucky, inspecting him. They notice a sore on his neck and begin to ask him a question, but Pozzo tells them to leave him alone.
Estragon asks Pozzo if he can have the bones from his chicken, and Pozzo tells him that Lucky gets priority over them. Estragon asks Lucky if he wants the bones, but he does not reply, and Pozzo tells Estragon that he can have the bones. He comments that he has never known Lucky to refuse a bone and hopes that he is not sick.
Vladimir suddenly explodes with anger at Pozzo's treatment of Lucky, but then seems embarrassed at his outburst. Pozzo decides to go, but then decides to stay and smoke another pipe. Vladimir wants to leave, but Pozzo reminds him of his appointment with Godot.
Estragon begins to wonder aloud why Lucky does not put down his bags. Pozzo begins to answer the question, after much preparation involving his vaporizer spray, but gives a convoluted and contradictory response. Vladimir asks Pozzo if he wants to get rid of Lucky; Pozzo responds that he does and is taking him to the fair to sell him.
Lucky begins to cry, and Pozzo hands Estragon a handkerchief to wipe away his tears. Estragon approaches Lucky, but Lucky kicks him in the shins. Pozzo tells Vladimir and Estragon that he has learned a lot from Lucky, and that Lucky has been serving him for nearly sixty years. Vladimir becomes angry that Pozzo is going to get rid of Lucky after so much time, and Pozzo gets upset. Vladimir then gets angry at Lucky for mistreating Pozzo.
Pozzo calms down, but he realizes that he has lost his pipe and begins to get upset again. While Estragon laughs at Pozzo, Vladimir exits, apparently to go to the bathroom. He returns, in a bad mood, but soon calms down. Pozzo sits down again and begins to explain the twilight. When he finishes, he asks them to evaluate his performance and then offers to have Lucky perform for them. Estragon wants to see Lucky dance, while Vladimir wants to hear him think, so Pozzo commands him to dance and then think.